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P192. The dynamics of B-lymphocytes before and after transplantation of allogeneic mesenchymal stromal cells of bone marrow in patients with ulcerative colitis

L. Lazebnik1, O. Knyazev1, M. Yakovleva2, T. Astrelina2

1Central Scientific Research Institute of Gastroenterology, Moscow, Russian Federation; 2Stem Cell Bank, Moscow, Russian Federation

In the development of inflammation in ulcerative colitis (UC) involving numerous mechanisms of tissue and cell damage. Bacterial and tissue antigens causing stimulation of T-and B-lymphocytes. In ulcerative colitis increases the population of B-lymphocyte and plasma cell and the subsequent release of inflammatory mediators, which cause destruction of the epithelial cells of the mucous membrane of the colon.

Aim: To evaluate changes in populations of B-lymphocytes before and after transplantation of allogeneic mesenchymal stromal cells (MSCs) of bone marrow in patients with UC.

Material and Methods: Systemic MSC transplantation performed patients c chronic continuous and chronic recurrent course of UC. Seven patients with UC in the peripheral blood before and 1 month after transplantation of MSCs was determined parameters of cellular immunity (the number of CD19, CD19 CD5, CD19 CD5−, CD19 CD10) by flow cytometry on cytometry.

Results: Prior to transplantation of MSCs in patients with UC in the period of exacerbation the absolute content of B-lymphocytes (CD19) was 0.26±0.06 109/l, the content of autoreactive clones of B-1 cells (CD19 CD5) – 0.04±0.01 109/l, B-2 lymphocytes (CD19 CD5−) – 0.2±0.05 109/l and B-cell precursors (CD19 CD10) – 0.04±0.01 109/l. After the MSCs transplantation the absolute content of B-lymphocytes in patients with UC in the study group, almost unchanged and amounted to 0.27±0.06 109/l, significantly decreased the absolute content of autoreactive clones of lymphocytes in-l to 0.02±0.007 109/l (p < 0.05), the contents of B-2 cells was 0.24±0.05 109/l, the contents of B-cell precursors tended to decrease and was in absolute values of 0.03±0.008 109/l (p > 0.05). Significant decrease in the content of autoreactive clones of B-1 lymphocytes indicates a decrease in the level of autoaggression organism.

Conclusion. Transplantation of MSCs leads to a decrease in the activity of autoimmune processes in patients with ulcerative colitis, which leads to the endoscopic and clinical remission.