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P049. Mesalazine and prednisolone can ameliorate the harmful effects of per os administration of iron in experimental colitis in rats, while the biologic agents infliximab and adalimumab can not

J.K. Triantafillidis1, G. Douvi2, A. Papalois3, G. Agrogiannis4, A. Gikas2

1“St Panteleimon General Hospital, Nicea, Greece; 2“St Panteleimon” General Hospital, Nicea, Greece; 3Experimental-Research Center, ELPEN Pharma, Athens, Greece; 4A' Department of Pathology, University of Athens, Athens, Greece

Aim: To investigate the influence of mesalazine, prednisolone, infliximab and adalimumab on the degree of colonic inflammation induced by oral iron administration in experimental colitis in rats.

Materials and Methods: Colitis was induced by TNBS. Iron was administered via a mouth catheter at a dose of 0.027 mg/d (normal diet), 0.3 g (1.9 ml), and 3 g (9 ml)/d respectively. Mesalazine and prednisolone were administered via a mouth catheter at a dose of 30 and 1 mg/kg bw/d respectively. Infliximab and adalimumab were administered sc on the 2nd and 6th day and on the 2nd day respectively, at a dose of 5 and 1 mg/kg bw respectively. 252 Wistar rats were allocated into 18 groups of 14 animals, as follows: On the 8th day, all animals were killed. Activity of colitis and extent of tissue damage was assessed histologically.

Results: Significant differences in all histological parameters examined were noticed between group 1 vs group 2 and 3 (P < 0.002), between group 4 vs. groups 5 and 6 (P < 0.001), between group 2 vs. groups 7 and 9 (P = 0.006 and 0.035, respectively), group 3 vs. group 10 (P = 0.032), group 5 vs. groups 11 and 13 (P = 0.00001 and 0.042, respectively), between group 6 vs groups 12 and 14 (P = 0.0001, both), between group 5 vs. 13 (P = 0.00001) and between group 6 vs. groups 15 and 13 (P = 0.0001 and 0.001, respectively).

Conclusion: Iron can induce colonic inflammation and aggravate TNBS colitis. Mesalazine and prednisolone ameliorated the inflammatory process in the colonic mucosa induced by administration of oral iron in TNBS colitis, while infliximab and adalimumab did not.

Acknowledgments: This work was supported by the Hellenic Society of Gastroenterology.

GroupTNBSIron dietIron 0.3 gIron 3 gMesalazinePrednizoloneInfliximabAdalimumab
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2+
3+
4++
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9++
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11++
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18+++