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P061. IFN-γ release assay in screening for latent tuberculosis in inflammatory bowel diseases

P. Papay, A. Eser, S. Winkler, S. Frantal, C. Primas, W. Miehsler, S. Angelberger, G. Novacek, H. Vogelsang, W. Reinisch

MUW Vienna Austria, Vienna, Austria

Background: IFN-γ release assays (IGRA), widely used for latent tuberculosis screening prior to anti-TNF-α treatment, are limited by indeterminate results in patients under immunomodulatory (IM) therapy. The aim of our study was to delineate factors associated with indeterminate IGRA results.

Methods: A total of 190 patients with inflammatory bowel disease were included. IGRA was indeterminate if the result of IFN-γ concentration was <0.35 IU/ml for tuberculosis-specific antigens and <0.5 IU/ml for the positive control. Predictors for indeterminate results were delineated from multivariate logistic regression.

Results: IGRA was indeterminate in 26/190 (13.7%) patients. Indeterminate IGRA were associated with lower serum albumin levels (odds ratio [OR] 0.88, 95% confidence interval [CI] 0.79–0.96), lower absolute lymphocyte count (OR 0.39, 95% CI 0.18–0.75) and double IM therapy (OR 2.98, 95% CI 0.95–8.90). Sub-analysis of IM therapy revealed an association of steroid therapy with indeterminate IGRA (OR 3.19, 95% CI 1.35–7.70). Hypoalbuminaemia increased the risk of indeterminate IGRA by (OR 2.97, 95% CI 1.03–8.61) and lymphopaenia by (OR 3.28, 95% CI 1.41–7.65). After a mean of 18.5±14.4 days retesting of IGRA in 18 patients with indeterminate results yielded 9 negative versus 9 indeterminate results.

Conclusion: Our results reveal associations of indeterminate IGRA with low serum albumin levels and absolute lymphocyte count and double IM therapy. IGRA testing appears best to be performed prior to initiation of IM therapy in patients with inflammatory bowel disease.