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P078. The first experience in marked mesenchymal stromal cell in Crohn's disease

L. Lazebnik1, A. Konoplyannikov2, O. Knyazev1, A. Parfenov1

1Central Scientific Research Institute of Gastroenterology, Moscow, Russian Federation; 2Medical Radiological Research Center of Russian Academy of Medical Sciences, Obninsk, Russian Federation

Until the recent time there were only theoretical grounds of tropism of Mesenchymal Stromal Cell (MSC) to inflamatory focus. Were not direct cell labeling techniques (introduction of imaging marker, followed by imaging of its accumulation in inflammatory)

Aim: Show physiological and pathological accumulation of marked MSC in organs and tissues with Crohn's disease (CD).

Materials and Methods: Intravenous infusion stream type of infusion of 10 ml of saline with 20×106 MSC, marked with Tc99m-HMPAO (Ceretec) were done to CD patient. Result estimation was held in 5 min, 1 hour, 3 hour and 16 hours after MSC infusion. Estimation was held with multiplanar imaging scintigraphy of the entire body (rotating gamma camera “Sophy-camera”). Scintigrams were visually estimated. Results were interpreted with the adjustment of negligible capture of lipophilic molecules Tc99m-HMPAO in brain tissues. The rest part of Tc99m were excreted with kidneys and liver and therefore this organs were visualized as well. Urinary tracts, urinary bladder, gallbladder and bowels were visualized too.

Results: Multiplanar imaging of the entire body of the patens with Crohn's disease were held in 5 min, 1 hour, 3 hour and 16 hours after MSC infusion. Activity was less than 350 MBk. At the moment of infusion (15–30 sec) was registered physiological accumulation of MSC in lungs (it is typifying to starting allocation of stromal cells). On the early scintigrams (in 5 minutes and in 1 hour) pathological accumulation in the walls of all areas of the large intestine was registered (selective painting of inflammatory area). In 3 hours after infusion accumulation was registered in liver, gallbladder and bowels (it is due to excretion of detached mark). In 16 hours after infusion accumulation was registered in Urinary tracts and kidneys (it is due to excretion of detached mark). Pathological fixation of mesenchymal sromal cells in CD patients was registered in large intestine (in organs and tissues, where is increased excretion of anti-inflammatory cytokines). But it is still impossible to estimate long-term relocation of cells, due to Tc99m properties, that is used for cell radiolabeling. Also this makes difficult in estimation of allocation of stem cells in liver, bowels and urinary organs.

Conclusion: Pathological fixation of mesenchymal sromal cells in CD patients was registered in large intestine (in organs and tissues, where is increased excretion of anti-inflammatory cytokines). But it is still impossible to estimate long-term relocation of cells, due to Tc99m properties, that is used for cell radiolabeling. Also this makes difficult in estimation of allocation of stem cells in liver, bowels and urinary organs.