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P022. Treatment with CSF and GSF growth factors improves experimental colitis in rats

H. Triantafillidis1, A. Papalois2, K. Barbatis3, J. Loukopoulos1, A. Gikas1, J. Triantafillidis1

1“Saint Panteleimon” Hospital, Gastroenterology, Nicea, Greece; 2Research Laboratory ELPEN Pharmaceutical Company, Athens, Greece; 3Department of Pathology Red Cross Hospital, Pathology, Athens, Greece

Background: There are some indications suggesting that treatment with growth factors could have beneficial effect in both, patients with inflammatory bowel disease and experimental colitis. Oxidative stress is an essential factor in the pathophysiology of experimental colitis. The aim of this study was to investigate the influence of two Growth factors, namely Colony Stimulating Factor (CSF), and rHu Granulocyte Stimulating Factor (GSF) in experimental colitis in rats.

Methods: Chemical colitis was induced in 62 male Wistar rats (divided into 9 groups) using 2,4,6-trinitrobenzensulfonic acid (TNBS)

Groups of animals and treatment
GroupNumber of animals with TNBS colitisDay of euthanasiaTreatment with CSFTreatment with rHu GSFTreatment with prednisolone
110 (control group, No treatment)after 15 days  
210 (control group, No treatment)after 30 days  
36after 19 daysYes 
46after 19 daysYes, 7 days after 
56after 19 daysYes, 14 days after 
66after 19 days Yes
76after 19 days Yes, 7 days after induction of colitis
86  Yes, 14 days after induction of colitis
96after 15 days  immediate treatment with prednisolone

Growth factors were administered sc every 2 days at a dose of 10 mcg/Kg. The dose of prednisolone was 5.3×10×3 mmol/Kg. Histological changes of colonic tissues (histological score) were examined by hematoxylin and eosin staining. In addition, malondialdehyde (MDA) content in the homogenate was measured.

Results: The administration of CSF improved significantly the histological score, compared to control groups in all animals receiving this drug (P < 0.01), although results concerning group 5 were less impressive compared with groups 3 and 4. The administration of GM-CSF improved significantly the histological score in all groups receiving this drug, although in a lesser degree concerning the group of delayed administration. Comparison of the two growth factors revealed that CSF was superior to GM-CSF especially in the groups with delayed administration. Interestingly enough, differences between CSF and prednisolone were statistically significant in favor of CSF (P < 0.05). MDA contents decreased accordingly in groups receiving these drugs compared with controls (P < 0.01).

Conclusions: The administration of CSF and GM-CSF can significantly improve the histological score and oxidative stress in experimentally induced colitis in rats. Early administration produces even better results.