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P219. Effect of mesenchymal stem cells to the level of adhesion molecules in patients with inflammatory bowel disease

L. Lazebnik1, O. Boldyreva1, O. Knyazev1, V. Sagynbaeva1

1Central Research Institute of Gastroenterology, Moscow, Russian Federation

Background: Intercellular adhesion molecule provide the mechanical interaction of cells with each other. Increased adhesiveness of great importance in the pathogenesis of endothelial dysfunction during inflammation, atherosclerosis, septic shock and other pathological processes.

Aim: To study the effect of mesenchymal stem cells (MSCs) of bone marrow to the level of adhesion molecules in patients with inflammatory bowel disease (IBD).

Methods: We examined 14 patients IBD who received MSCs culture, including 9 patients with ulcerative colitis (UC) and Crohn's disease in 5 patients (CD). Age of patients ranged from 20 to 58 years, average age, 35, 6±3.0 (M±m). The diagnosis of UC and BC data confirmed the clinical, laboratory, histological, and instrumental methods. Before and after the transplantation of MSCs to determine the level of adhesion molecules L‑selectin, E‑selectin, P‑selectin, sVCAM‑1 in serum by ELISA using test-systems “Bender MedSystems” (Austria).

Results: L‑selectin level before beginning treatment MSCs in serum of patients with IBD was 9.05±0.5 ng/ml, E‑selectin – 10.1±3.3 ng/ml, P‑selectin – 12.2±1.8 ng/ml, sVCAM‑1 – 13.9±4.4 ng/ml. The level of SP-selectin, E‑selectin after transplantation of MSCs significantly decreased to 6.6±1.1 ng/ml, 5.7±1.3 ng/ml, respectively (p < 0.001). The concentration of sL-selectin and sVCAM‑1 after administration of MSCs in the serum was 8.9±0.5 ng/ml (p = 0.6), 12.2±2.2 ng/ml (p = 0.2).

Conclusions: Thus, as a result of the study mesenchymal stem cells in bone marrow significantly reduces the level of P‑selectin, E‑selectin, but does not affect the concentration of L‑selectin and sVCAM‑1 in patients with inflammatory bowel disease.