Search in the Abstract Database

Search Abstracts 2013

* = Presenting author

P065. Humoral immune response to intestinal microbiota in inflammatory bowel diseases

D. Abdulganieva1, O. Zinkevich2, N. Saphina2, D. Mukhametova1, A. Odintsova3, 1Kazan State Medical University, Kazan, Russian Federation, 2Kazan State Medical Academy, Kazan, Russian Federation, 3Republican Clinical Hospital, Kazan, Russian Federation

Background

Evidence suggests that inflammatory bowel disease (IBD) results from an inappropriate inflammatory response to intestinal microbes in a genetically susceptible host.

Methods

22 pts with IBD exacerbation (15 ulcerative colitis [UC], 7 Crohn's disease [CD]) and 15 healthy controls were included into the study. IgM and IgG to lipopolysaccharide (Lps) of E. coli O14, protein antigens of E. coli M 17, P. aeruginosa, P. mirabilis, C. albicans, K. pneumoniae, Strep. spp., S. aureus were evaluated by immunoassay. Mean age in UC was 36.1±6.4 years, CD – 37.4±6, controls – 30.4±6.6.

Results

There was decreasing of IgM and IgG to majority of antigens in UC and CD compared to controls (Tables 1, 2). IgM to Strept. spp. was increased in UC (p < 0.05), IgG to Lps of E. coli O14 was decreased in CD (p < 0.05). Humoral immunity correlated with IBD courses: in UC IgM to Lps of E. coli O14 and distribution of the disease (r = 0.6; p < 0.05), in CD – IgM to Strept. spp. (r = 0.8; p < 0.05), IgG to P. mirabilis (r = 0.7; p < 0.05) and disease duration. Some associations with clinical parameters were showed: bloody diarrhea was associated in UC with IgM to Lps of E. coli O14 (r = −0.6; p < 0.05), IgG to P. mirabilis (r = 0.6; p < 0.05) and in CD with IgM to S. aureus (r = 0.8; p < 0.05), IgG to P. mirabilis (r = −0.9; p < 0.05). The abdominal distention correlated in CD with IgM to C. albicans (r = 0.8; p < 0.05) and with IgG to Lps of E. coli O14 (r = 0.7; p < 0.05).

Table 1
IgMUC, n (%)CD, n (%)
 normnorm
Lps of E. coli O147 (46.7)3 (20)5 (33.3)5 (71.4)1 (14.3)1 (14.3)
Protein antigens of E. coli M 179 (60)5 (33.3)1 (6.7)4 (57.1)3 (42.9)0
P. aeruginosa7 (46.7)4 (26.6)4 (26.7)3 (42.9)3 (42.9)1 (14.3)
P. mirabilis4 (26.7)9 (60)2 (13.3)3 (42.9)3 (42.9)1 (14.3)
C. albicans6 (40)8 (53.3)1 (6.7)4 (57.1)2 (28.6)1 (14.3)
K. pneumoniae2 (13.3)9 (60)4 (26.7)2 (28.6)4 (57.1)1 (14.3)
Strep. spp.1 (6.7)4 (26.6)10 (66.7)3 (42.8)1 (14.4)3 (42.8)
S. aureus2 (13.3)6 (40)7 (46.7)1 (14.4)3 (42.8)3 (42.8)
Total5 (33.3)6 (40)4 (26.7)3 (45)2.5 (36)1.5 (15)
Table 2
IgGUC, n (%)CD, n (%)
 normnorm
LPS of E. coli O147 (46.7)7 (46.7)1 (6.6)4 (57.1)3 (42.9)0
P. aeruginosa11 (73.3)4 (26.7)04 (57.1)3 (42.9)0
P. mirabilis7 (46.7)4 (26.7)4 (26.7)4 (57.1)2 (28.6)1 (14.3)
C. albicans9 (60)6 (40)05 (71.4)1 (14.3)1 (14.3)
K. pneumoniae1 (6.6)7 (46.7)7 (46.7)04 (57.1)3 (42.9)
Total7 (46.7)5.6 (37.3)2.4 (16)3.4 (50)2.6 (38)0.8 (12)

Conclusion

The majority of pts with IBD have decreased IgM and IgG to intestinal microbes compared to control. IM changes correlated with IBD course and clinical features.