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P010. The influence of vitamin D3 for colitis model of rats

W. Tang1, Y.-J. Ge2, 1The Second Affiliated Hospital of Soochow University, Gastroenterology, SanXiangLu, Suzhou, 215004, China, 2The second Affiliated Hospital of Soochow University, Gastroenterology, Suzhou, China

Background

Immunomodulatory effects of vitamin D (VitD) on inflammatory bowel disease(IBD) has attracted more and more attention.

Aims: To investigate the effect of VitD3 on colitis induced by TNBS in rats.

Methods

Sixty-six male rats were randomly divided into 5 groups: blank control group, TNBS group, different doses VitD3. All the rats except blank controls were given TNBS enema to induce colitis model. After 24 hours, drug intervention by gastric gavage was performed. All rats were sacrificed on the 10th day. Disease activity index (DAI), macroscopic injury and histological score were evaluated, the activity of caspase-3, TNF-a, CD40 were detected by RT-PCR. Serum calcium and creatinine levels were determined to monitor side effect.

Results

Compared with TNBS group, macroscopic injury score and histological score in single high dose and continuous high dose VitD3 were significantly decreased (P < 0.05); the activity of caspase-3, TNF-a, CD40 in all drug intervention groups were significantly decreased (P < 0.01); no significant difference in mRNA expression was found between TNBS group and all drug intervention groups. Rats in continuous high dose VitD3 group had hyperealcemia and serum level of creatinine was significantly increased in continuous high dose VitD3 group when compared with the other groups (P < 0.05).

Conclusion

VitD3 can alleviate TNBS induced experimental colitis by regulating T cell immunity.