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P012. The effects of mesenchymal stem cells as a cell therapy in animal model of Crohn's disease

B.I. Jang1, W.Y. Jung2, I.H. Song2, K.O. Kim1, C.H. Yang3, G.A. Song4, 1Yeungnam University College of Medicine, Division of Gastroenterology and Hepatology, Department of Internal Medicine, Daegu, Korea, Republic of, 2Yeungnam University College of Medicine, Department of Anatomy, Daegu, Korea, Republic of, 3Dongguk University College of Medicine, Division of gastroenterology and Hepatology, department of Internal Medicine, Kyungju, Korea, Republic of, 4Pusan National University School of Medicine, and Biomedical Research Institute, Division of Gastroenterology and Hepatology, Department of Internal Medicine, Busan, Korea, Republic of

Background

Inflammatory bowel disease (IBD) is a complex immunological disease characterized by chronic inflammation with unknown cause. Interleukin-10 Knockout (IL-10 KO) mouse is a well-established murine model of IBD which develop a spontaneous intestinal inflammation that resemble with Crohn's disease.

In the present study, human adipocyte derived stem cells (hAMSC) were administrated to IL-10 KO mice to find out anti-inflammatory effects of hAMSCs may attenuate progress or treat of IBD.

Methods

IBD was induced in IL-10 KO mouse by feed piroxicam mixed diet for 1 week and then, 2×106 hAMSC were injected into peritoneum and changed to normal diet. After 1 week, the mice were sacrificed and tissue sample was harvested and tissue score for inflammation and inflammation related gene expression were evaluated.

Results

hAMSC administrated group showed reduced inflammatory changes significantly. The effect was more powerful in ascending colon than descending or cecum in regional analysis. In categorical analysis, protection effect on chronic inflammation was more evident than acute inflammation considering ordinary progress of IBD; inflammatory cells appear, goblet cells change, mucosa thickening, submucosa cell infiltration, and destruction of architecture. RT-PCR analysis showed that Rantes, toll like receptor, IL-4 expression were not noticeably different between groups but IL-12, INF, TNF-a showed significant decrease in hAMSC group.

Conclusion

hAMSC attenuate IBD induction in IL-10 KO mouse by suppression of inflammatory cytokine expression and this expected to be influenced through type 1 helper T cell pathway. Even though single injection of hAMSC, the effect influenced chronic event of inflammatory changes. These findings suggested that administration of hAMSCs show beneficial effect on animal model of CD and emerge a therapeutic option in inflammatory bowel disease.