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P022. Role of P2X7 purinergic receptor in the control of enteric neuromuscular functions in normal rat distal colon and experimental bowel inflammation

L. Antonioli1, M. Fornai1, R. Colucci1, C. Pellegrini1, D. Sacco1, V. Caputi2, G. Orso3, M.C. Giron2, C. Scarpignato4, C. Blandizzi1, 1University of Pisa, Department of Clinical and Experimental Medicine, Pisa, Italy, 2University of Padova, Department of Pharmaceutical and Pharmacological Sciences, Padova, Italy, 3Scientific Institute “E. Medea”, Scientific Institute “E. Medea”, Padova, Italy, 4University of Parma, Laboratory of Clinical Pharmacology, Parma, Italy

Background

Recent evidence supports an active involvement of the purinergic P2X7 receptor (P2X7R) in the fine tuning of immune functions, as well as its critical role in driving enteric neuron apoptosis under intestinal inflammation. However, the participation of this receptor pathway in the regulation of enteric neuromuscular functions remains undetermined. This study was aimed at investigating the role of P2X7Rs in the control of colonic motility, both under normal conditions and in the presence of experimental colitis.

Methods

Colitis was induced by intrarectal administration of 2,4-dinitro-benzene-sulfonic acid (DNBS) in adult male Sprague-Dawley rats. Six days after colitis induction, colonic longitudinal muscle strips (LMS), obtained from normal or inflamed rats, were suspended in organ baths, containing Krebs solution additioned with NK 1, 2 and 3 receptor antagonists, and connected to isometric transducers to record atropine-sensitive cholinergic motor activity. The effects of A804598 (selective P2X7R antagonist; 0.001–100 µM) and BzATP (selective P2X7R agonist; 0.001–10 µM) were tested on contractions evoked by electrical stimulation (ES: 0.5 ms, 28 V, 10 Hz), delivered as single train (sES) or repeated every 60 s (rES), or by carbachol (1 µM) in the presence of tetrodotoxin.

Results

In normal LMS, A804598 induced a negligible enhancing effect on sES-induced contractions (+7.8±3.5% at 0.1 µM), while a significant increase in the contractile responses elicited by sES was recorded in LMS from inflamed animals (+42.5±3.9% at 0.1 µM). Incubation of LMS with the adrenergic blocker guanethidine (10 µM) did not affect the enhancing effect exerted by A804598 in the presence of colitis. In the presence of N ω-propyl-L-arginine (NPA, inhibitor of neuronal nitric oxide synthase), which per se increased the sES-induced contractions in both LMS preparations (+15.1±5.5% in normal and +143.5±9.5% in inflamed animals), the A804598 effects were lost. The pharmacological activation of P2X7Rs with BzATP did not significantly affect the contractions to rES in normal LMS (Emax= −10.2±1.9%), while a marked reduction was recorded in LMS from animals with colitis (Emax= −30.5±2.2%). The inhibitory effect of BzATP was antagonized by A804598, and it was also markedly blunted by NPA. Both P2X7R ligands did not affect carbachol-induced contractions.

Conclusion

The purinergic system contributes to functional neuromuscular changes associated with bowel inflammation through the involvement of P2X7Rs, which modulate the activity of excitatory cholinergic nerves via a facilitatory control on inhibitory nitrergic pathways.