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P078. Effect of methanolic bark extracts of Chrysophyllum albidum on acetic acid-induced colitis in rats

A.T. Salami, A.I. Oyelami, T.P. Omayone, E.O. Adewoye, S.B. Olaleye, University of Ibadan, Physiology, Ibadan, Nigeria

Background

The bark of Chrysophyllum albidum has been reported to possess beneficial pharmacological properties. Previous reports show its antiplasmodial, haematinic, antimicrobial and kill kinetics properties. This study was designed to evaluate the healing and anti-inflammatory properties of methanolic extract of dried barks of Chrysophyllum albidum (sapotacea) (MeCaB) and chromatographic fractions (CFr A, B and C) on acetic acid induced colitis in rats.

Methods

Male albino rats ( = 10) were pre-treated with MeCaB (250 and 500 mg/kg p.o) and CFr A, B and C (120 mg/kg p.o) for 3 consecutive days before acetic acid intra rectal administration and was continued for 7 days after induction. The positive and negative control groups received 0.4mls of distilled water (vehicle) daily. Physical changes (Mucous/blood Stool score, body weight changes) colon weight to length ratio, haematological parameters, macroscopic (Ulcer) score, histological changes, colon tissue antioxidant (catalase, CAT), protein levels and free radical (lipid peroxidation, MDA) activities were recorded for days 3 and 7 post colitis induction.

Results

All the parameters (especially physical changes and haematology) were altered on colitis induction in each group. Treatment with MeCaB, CFr A, B and C by days 3 and 7 post colitis induction significantly improved all the physical changes, colon weight to length ratio, haematological parameters, macroscopic scores and histological changes as well as the CAT, protein and MDA levels compared with colitis untreated or normal groups.

Conclusion

Chrysophyllum albidum and its chromatographic fractions (especially 250 mg/kg MeCaB and CFr A) possess potent therapeutic and healing efficacy possibly by its antioxidant and anti-inflammatory properties. Work is ongoing to determine the mechanism of action by which this plant modulates acetic acid-induced colitis.