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P103. Usefulness of fluorescence spectroscopy in the diagnosis of inflammatory bowel diseases

M. Klopocka1, A. Liebert1, E. Kaluzna2, M. Manerowski1, J. Fisz2, J. Kubica3, 1Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Department of Gastroenterology Nursing, Bydgoszcz, Poland, 2Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Department of Informatics and Methodology of Scientific Work, Laboratory of Biomedical Photonics, Bydgoszcz, Poland, 3Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Department of Cardiology and Internal Medicine, Bydgoszcz, Poland

Background

Differential diagnosis in some cases of inflammatory bowel diseases is still an unsolved problem that requires development of sensitive and specific diagnostic methods. The same applies to factors predictive of the disease course. The usefulness of fluorescence-based research has already been reported as a potential new method for assessing intestinal tissue [1]. It is believed that the changes in the environment of natural fluorophores located in intestinal tissue during the course of the disease may influence the spectral properties of the tissue [2, 3].

The aim of the study was to measure spectroscopic features of intestinal tissue collected from Crohn's disease (CD) and ulcerative colitis (UC) patients.

Methods

Emission spectra of intestinal tissue collected during the diagnostic process from patients with CD, UC and healthy controls were recorded with a spectrofluorimeter Hitachi F-7000 (Japan). Emission spectrum was recorded in the wavelength range from 200 to 800 nm. In order to determine the emission properties of intestinal tissue, the excitation light in the wavelength range from 230 to 410 nm was used. Light wavelength was changed by every 20 nm. The fluorescence emission spectra were analyzed using the program ORIGIN 9.0 (OriginLab).

Results

Qualitative differences (different shapes of the emission spectra) were observed between the intestinal tissue collected from patients with CD, UC and healthy controls, arising from the images with excitation light having a wavelength of 250 and 310 nm. There were also quantitative differences of the fluorescence intensity between the intestinal tissue from patients suffering from CD and UC when the excitation light had a wavelength of 290 nm.

Conclusion

Preliminary results indicate on the qualitative and quantitative differences between the spectra of fluorescence of intestinal tissue samples taken from patients with CD, UC and healthy controls. It is therefore possible to observe changes in the spectra which are unique to a particular disease entity. Based on our results and literature data it can be concluded that fluorescence spectroscopy could be used as a valuable complementary method in the diagnosis of intestinal diseases. Introduction of new non-invasive diagnostic method can help to reduce the time and cost of the diagnostic process, and choose the optimal method of treatment.

1. Bottiroli G et al, (1995), Natural fluorescence of normal and neoplastic human colon

2. DaCosta RS et al, (2003), Molecular fluorescence excitation-emission matrices relevant to tissue spectroscopy

3. DaCosta RS et al, (2007), Fluorescence and spectral imaging.