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P169. Plasma biomarkers of endothelial dysfunction: monocyte chemoattractant protein 1 (MCP 1) and soluble CD40 ligand (CD40L) in patients with inflammatory bowel diseases

D. Cibor, D. Owczarek, A. Ciesla, M. Szczepanek, M. Glowacki, T. Mach, Jagiellonian University Medical College, Cracow, Poland, Gastroenterology, Hepatology and Infectious Diseases, Cracow, Poland

Background

One of the factors of a high importance in development of IBD is endothelial dysfunction. As a result of enhanced proliferation and activity of endothelial cells, intestinal microcirculation is altered, leading to local disturbances of intestinal blood supply with subsequent development of mucosal ulcerations. Intestinal microcirculation has an influence on number and kind of migrating to intracellular space leucocytes. High soluble CD40 ligand levels (sCD40L) are associated with increased expression of adhesion molecules and monocyte chemotactic protein (MCP-1) along with impaired endothelial cell migration and enhanced superoxide generation by monocytes.

The study aimed to assess level of endothelium dysfunction markers MCP 1 and CD40L in patients with IBD.

Methods

Eighty patients aged 18–70 with diagnosed inflammatory bowel disease [40 consecutive patients with Crohn's disease (CD) and 40 consecutive patients with ulcerative colitis (UC)] were included into the study. Levels of MCP 1 and CD40 L were measured in all patients and related to disease phenotype, clinical and biochemical activity.

Results

In UC group (22F and 18M) mean age was 36±11.7 years, in CD group (19F and 21M) mean age was 32±10 years. We have not found any statistical differences between UC and CD groups: mean level of sCD40 in UC group was 402±484 pg/ml and in CD 512±660 pg/ml (p = 0.2); mean level of MCP 1 in UC group was 139±108 pg/ml and in CD 159±96 pg/ml (p = 0.25). Level of CD40L was elevated in 28 patients with UC (70%) and in 32 patients with CD (80%). MCP 1 level was elevated only in 2 patients with UC (5%) and in 3 patients with CD (7.5%). sCD40L level correlated positively with CD activity (r = 0.32, p < 0.05). MCP 1 also correlated positively only with CD activity (r = 0.32, p < 0.05).

Conclusion

In patients with IBD sCD40L level is increased what might indicate on endothelial dysfunction in this group of patients. sCD40L correlates positively with both CD and UC activity. Farther studies are needed weather it might be used as a marker of disease activity.