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P185. Mucin 16 (MUC16) and mucin 20 (MUC20) over-expression in colonic mucosa is associated with histological remission in patients with ulcerative colitis

J. Yamamoto-Furusho1, I. Ascaño-Gutiérrez1, J. Furuzawa-Carballeda2, G. Fonseca-Camarillo1, 1IBD Clinic, Instituto Nacional de Ciencias Medicas y Nutricion, Gastroenterology, Mexico, Mexico, 2Instituto Nacional de Ciencias Medicas y Nutricion, Immunology, Mexico, Mexico

Background

Patients with Ulcerative Colitis (UC) present a defect in the secretion and maintenance of the mucosal barrier as a result of inadequate expression of mucin genes (MUC) which forms part of the particular chronic inflammatory process of this disease. The aim of the present study was to determine the gene and protein expression of MUC16 and MUC20 in colonic tissue from patients with UC and controls.

Methods

We included a total of 80 patients with confirmed diagnosis of UC and were divided into three groups: 1) 40 active UC; 2) 40 remission UC and 3) 40 controls without endoscopic evidence of any type of colitis (infectious, post radiation, ischemic) or neoplasia. The relative quantification of the gene expression was performed by real time PCR (RT-PCR) for MUC16 and MUC20 genes and the gen GAPDH was used as reference. The protein expression was detected by immunohistochemistry with specific antibodies for MUC16 and MUC20. Statistical analysis was done using SPSS v17 program. A P value <0.05 was considered as significant.

Results

A total of 80 patients with UC (39 men and 41 women with a mean age of 42 years) and 40 controls (11 men and 29 women with a mean age of 49 years) were evaluated. The MUC16 gene expression was significantly increased in the UC remission group compared to the control group (P = 0.03). No association of MUC16 gene expression with clinical features was found. The MUC20 gene expression was found significantly decreased in patients with active UC compared to both in remission (P = 0.0001) and controls (P = 0.0001). The MUC20 gene expression was increased and it was associated with the presence of histological remission in patients with UC (P = 0.003, OR = 0.37). The Immunohistochemical analysis confirmed an important decreased production of MUC16 and MUC20 in patients with active UC compared to the control group.

Conclusion

The expression of MUC16 and MUC20 mucins was increased in patients with remission UC, suggesting that this over-expression in the gene and protein expression are associated with histological remission in patients with UC.