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P260. Clinical usefulness of serum IL-32 in Crohn's disease

E.R. Kim1, S.H. Oh1, S.N. Hong1, S.-H. Kim2, D.K. Chang1, Y.-H. Kim1, 1Samsung Medical Center, Internal medicine, Seoul, Korea, Republic of, 2Konkuk University, Department of Biomedical Science, Seoul, Korea, Republic of

Background

IL-32 is a newly discovered cytokine and induces other proinflammatory cytokines including IL-1 beta, IL-6, TNF-alpha, and chemokines. Recent study demonstrated that IL-32 is overexpressed markedly in the inflamed tissues from patients with Crohn's disease (CD). We investigated the association with serum IL-32 titer and clinical manifestation of patients with CD, and identify whether serum IL-32 test is helpful in the differential diagnosis between CD and intestinal tuberculosis (ITB).

Methods

Serum samples from 48 patients with CD, 46 patients with ITB and 20 normal control were collected. Serum IL-32 gamma (most active isoform of IL-32) titer was measured by IL-32 gamma specific sandwich ELISA.

Results

Serum IL-32 gamma titer in patients with CD was significantly elevated compared with patients with ITB and normal control (p < 0.01). Between patients with ITB and normal control, serum IL-32 gamma titers were not significantly different. In patients with CD, serum IL-32 gamma titer tended to be increased patients with clinical symptoms such as weight loss, abdominal pain and hematochezia, and patients with lesion involved small bowel and anorectal area (p > 0.05). In patients with CD, serum IL-32 gamma titer of normal CRP group was higher than elevated CRP group, but there was no significant difference between two groups (p = 0.068).

The sensitivity, specificity, positive predictive value and negative predictive value of serum IL-32 gamma titier for diagnosis of CD were 64.6%, 73.9%, 45.7% and 54.3%, respectively.

Conclusion

Serum IL-32gamma titer can represent CD activity and be helpful in the differential diagnosis between CD and ITB. However, prospective large studies are needed to verify the clinical usefulness of serum IL-32gamma titer in diagnosis and monitoring of CD.