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P465. IL-4 in pathogenesis of systematic manifestations of ulcerative colitis

V. Pavlenko, N.Korablina, G.Kataganova, S. Aleksandrova, 1Stavropol State Medical Institute, Stavropol, Russian Federation, 2University, Medical, Stavropol, Russian Federation

Background

To investigate IL-4 production by mononuclear cells in patients with systematic manifestations ulcerative colitis (UC).

Methods

49 patients with UC were observed aged from 26 to 47. Best's reliability criteria was used. The first clinical group consisted of 17 patients, in which extra intestinal symptoms concerned with the activity of bowel inflammation (arthritis, autoimmune thyroiditis, autoimmune hepatitis, erythema nodosum, sclerosing cholangitis) and 6 patients, in which extra intestinal symptoms weren't concerned with the activity of bowel inflammation (psoriasis, vitiligo). Severity of UC was middle (16.3%) and high (30.6%). The second group consisted of 26 patients, in which there were no extraintestinal symptoms. MNC were extracted from heparinized blood, washed, standardized (2×106), incubated with E. coli lipopolysaccharide (LPS) (10 mg/ml). IL-4 was determined in cell supernatants by ELISA. Ten healthy volunteers made control group.

Results

Spontaneous (7.35±0.2 pg/2×106 cells) and LPS-stimulated (8.1±0.2 pg/2×106 cells) production of IL-4 by MNC in control group was higher than in UC patients, the lowest level was in the first clinical group (4.78±0.3 and 6.31±0.35 pg/2×106 cells) compared with the second group, p < 0.05.

Conclusion

Lowered IL-4 levels in patients with systematic manifestations of UC are probably concerned with the formation of autoimmune component of pathogenesis.