P033 Expression of aryl hydrocarbon receptors (AHR) in the condition of experimental acute ileitis and administrations simvastatin and antagonist of receptors in interlekin-1
A. Kamyshny*1, A. Zherebiatiev2
1ZSMU, Microbiology, Zaporozhye, Ukraine, 2Zaporozhye State Medical University, Microbiology, Zaporozhye , Ukraine
The aryl hydrocarbon receptor (AhR), a transcription factor activated by a large number of environmental agents, modulates the activity of immune and no immune cells in the gut, and may represent an important link between the environment and the immune perturbations which underlie the pathogenesis of inflammatory bowel disease. We studied the possibility of simvastatin and antagonist of receptors of interleukin-1 for pharmacological correction of acute ileitis in rats with a focus on the expression intensity studies of AhR with lymphocytes of small intestine.
Experiments were carried out on male Wistar rats aged 5-7 months (body mass 260-285 g). Rats were divided into four experimental groups: group 1-control; group 2-rats with indomethacin-induced ileitis; group 3-rats given Simvastatin; group 4-rats given antagonist of receptors of interleukin-1. For induction of an acute ileitis, rats received one subcutaneous dose of indomethacin (Sigma, 15 mg/kg).The AhR immunopositive lymphocytes were determined using an indirect immunofluorescence technique with using a monoclonal rat antibody (Santa Cruz Biotechnology).
We established that development of ileitis was accompanied with the change of amount of AhR + lymphocytes and the density of AhR in immunopositive cells. Drug administration during the development of experimental pathology was accompanied by changes in the expression of AhR and their density on lymphocytes.
These novel findings may help explain how Simvastatin and antagonist of receptors of interleukin-1 seemed to be beneficial in indomethacin-induced rat ileitis model through regulate mucosal immune responses and modulate AhR expression with lymphocytes of small intestine.