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* = Presenting author

P039 Role of the CARD family (CARD10, CARD11 and CARD14) in the colonic mucosa of patients with Ulcerative Colitis

J. Yamamoto-Furusho*, A. Sarmiento, G. Fonseca-Camarillo

IBD Clinic, Instituto Nacional de Ciencias Medicas y Nutricion, Gastroenterology, Mexico, Mexico

Background

Ulcerative Colitis (UC) is one of the two forms of Inflammatory Bowel Disease (IBD), whose multifactorial origin is well accepted but its etiology remains unknown. The gene NOD2 (CARD15) is the major one associated gene regarding the development of Crohn´s Disease (CD), and codifies for a cytoplasmic protein that is involved in the molecular mechanisms of CD. Other members of the CARD family include the CARD10, CARD11 and CARD14 are recently recognized genes that encode cytoplasmic proteins from the same family that play a role in the apoptotic signaling cascade and activation of Nuclear Factor kB.

Aim: To determine the expression of some members of the CARD family (CARD10, CARD11 and CARD14) in patients with UC and controls without inflammation.

Methods

A total of 183 individuals divided into 3 groups (63 patients with active UC, 60 patients with UC in remission and 60 controls without histologic inflammation). In all cases the diagnosis was confirmed by histopathology. Total ribonucleic acid (RNA) was extracted from intestinal tissue, subsequently complementary deoxyribonucleic acid (cDNA) was obtained by polymerase chain reaction (PCR). For each gene, relative quantification was made by real-time PCR. The statistical analysis was performed using SPSS version 17.0, using Kruskall Wallis non-parametric test, Spearman`s correlation, Fisher`s exact test and Odds Ratio (OR) in order to determine the strength of association. A P value < 0.05 was considered as significant.

Results

The CARD14 gene expression was significantly higher in the group with active UC compared to controls (P=0.008) and statistical trend of significance in the group of UC in remission (P=0.07). The low expression of CARD14 gene was associated with a more benign clinical course of UC, characterized by initial activity followed by long-term remission longer than 5 years (P=0.01, OR= 0.07, CI 95%: 0.007-0.70). No statistically significant differences were found in the CARD10 and CARD11 gene expression.

Conclusion

The CARD family might be involved in UC pathophysiology. The CARD14 gene expression was increased in patients with active UC and the low expression of CARD14 gene was associated with a benign clinical course characterized by long-term remission.