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P132 Could the cytokines concentration be a marker of IBD activity and be usefull in evaluation of IBD differentiation?

I. Ciecko-Michalska*1, I. Wierzbicka-Tutka2, M. Szczepanek3, D. Fedak4, T. Mach5

1Jagiellonian University Medical College, Department of Gastroenterology ang Hepatology, Krakow, Poland, 2University Hospital, Department of Gastroenterology and Hepatology, Krakow, Poland, 3Jagiellonian University Medical College, Department of Gastroenterology and Hepatolgy, Krakow, Poland, 4Jagiellonian University Medical College, Department of Clinical Biochemistry, Krakow, Poland, 5Jagiellonian University Medical College, Department of Gastroenterology and Hepatology, Krakow, Poland

Background

In inflammatory bowel disease (IBD) the imbalance between cytokines pro- and antinflammatory is observed.

The aim of this study was the assessment of IL10, IL6 and TNF alfa concentration usefulness in the evaluation of the activity of ulcerative colitis (UC) and Cohn's disease (CD).

Methods

35 patients diagnosed with UC (age 18-55), and 39 with CD (age 21-50) were examined. The control group (CG) consisted of 35 healthy volunteers (age 21-50). Diagnosis of the disease was confirmed by videocolonoscopy and histopathological evaluation of intestinal biopsies. Disease activity of UC was assessed according to the Mayo Scoring System and by the CDAI in CD patients. Among patients with UC 18 (51%) had severe, 14 (40%) moderate and 3 (9%) mild disease. Among patients with CD 7 (18%) was diagnosed with high, 27 (69%) moderate, and 5 (13%) with low activity of the disease. WBC, PLT, serum concentration of TNF alfa, IL-6 i IL-10 were determined. The Results of the study were statistically analyzed using the program R.

Results

The average concentration of TNF alfa in UC patients was: 14,3 (IQR=12,6), in CD: 12,6 (IQR=11,9), in theCG: 3,1 (IQR=1,7). The average concentration of IL6 in UC was: 19,6 (IQR=21), in CD: 10,8 (IQR=7,6), in CG : 3,2 (IQR=1,6). The average level of IL10 in UC was: 14,4 (IQR=5,9), in CD: 10,4 (IQR=9,3), in the CG: 3,3 (IQR=2,5). In the IBD TNF alfa, IL6 and IL10 concentration was significantly higher than in CG. However, IL10 was significantly higher in UC than CD. In patients with UC statistically significant positive correlation between the concentration of TNF alfa, IL-6 and IL-10 and and disease activity was noticed. There were no correlation between TNF alfa, IL6 and IL10 concentration and CD activity.

Conclusion

Determination of TNF alfa, IL6 and IL10 serum concentration can be used for noninvasive evaluation of inflammation activity in patients with UC. IL10 concentration may be helpful in differentiation of UC and CD.