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* = Presenting author

P136 Usefulness of serum IL-32 and IL-33 as a predictor of clinical course in Crohn's disease

E.R. Kim*, Y.K. Park, S.N. Hong, D.K. Chang, Y.-H. Kim

Samsung Medical Center, Internal medicine, Seoul, Korea, Republic of

Background

IL-32 and IL-33 are newly discovered cytokine. IL-32 induces other proinflammatory cytokines including IL-1β , IL-6 and TNF-α, and recent studies demonstrated that IL-32 is overexpressed markedly in the inflamed tissues from patients with Crohn's disease (CD). IL-33 is a new member of the IL-1 superfamily of cytokines and can function as a proinflammatory cytokine inducing T-helper cells type-2 of immune response. We investigated the association with serum IL-32 and IL-33 titer, and clinical activity of patients with CD, and identify whether serum IL-32 and IL-33 test are helpful to predict the clinical course in patients with CD.

Methods

Serial serum samples from 15 patients (12 men and 3women: median age 31 years, range 20-42 years) with CD were collected during follow up period from January 2012 to May 2013. Serum IL-32 and IL-33 with IL-1b, IL-6, IL-10 and TNF-α were measured by commercially available enzyme linked immunosorbent assay kits. Serum C-reactive protein (CRP) and Crohn's disease activity index (CDAI) were also assessed.

Results

Univariate linear regression analysis showed significant positive correlations between IL-32 and CDAI (r[correlation coefficient]=0.002, p=0.013) and significant negative correlation between IL-33 and CDAI (r = -0.002, p=0.015). The correlation between IL-32 and CDAI was remarkable in CD patients involving both small and large intestine (r = 0.003, p=0.000), while in CD patients involving only small intestine, this correlation was disappeared. On the contrary, the correlation between IL-33 and CDAI was maintained in CD patients involving only small intestine (r = -0.003, p=0.022), while this correlation was disappeared in CD patients involving both small and large intestine. Other proinflammatory cytokines didn't show any correlation with CDAI and. Serum IL-32 didn't show the correlation with serum CRP. However, in CD patients with involving only small intestine, serum IL-32 showed significant positive correlation with serum CRP (r = 0.061, p=0.014).

Conclusion

Serum IL-32 and IL-33 can represent CD activity and be helpful to predict in the clinical course in patients with CD. However, prospective large studies are needed to verify the usefulness of serum IL-32 and IL-33 in monitoring of clinical course in CD patients.