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P705 Identification of 10 regions associated with IBD in a non- Caucasian Moroccan Population

L. Amininejad*1, M. Elansary2, I. Hama3, I. Ratbi3, V. Muls4, M. Talib5, P. Closset6, M. Van Gossum4, I. Errabih7, E. Théâtre2, B. Charloteaux2, Y. Momozawa2, E. Quertinmont1, V. Wambacq1, L. Karim2, N. Cambisano2, N. Ahariz2, H. Ouazzani7, E. Louis8, M. Abramowicz9, J. Devière1, A. Van Gossum1, A. Sefiani3, W. Coppieters2, M. Georges2, D. Franchimont1

1Erasme Hospital, Université Libre de Bruxelles, Department of Gastroenterology, Hepatopancreatology and Digestive Oncology and Laboratory of Experimental Gastroenterology, Brussels, Belgium, 2Groupe Interdisciplinaire de génoprotéomique Appliquée (GIGA-R) and Faculty of veterinary medicine, University of Liège (B34), Unit of Animal Genomic, Liège, Belgium, 3Faculté de médecine et de pharmacie, Université de Mohammed V, Centre de Génomique humaine, Rabat, Morocco, 4Saint Pierre Hospital, Department of Gastroenterology, Brussels, Belgium, 5Brugmann Hospital, Department of Gastroenterology, Brussels, Belgium, 6Ixelles Hospital, Department of Gastroenterology, Brussels, Belgium, 7Avicenne Hospital, Department of Medecine B, Rabat, Morocco, 8Sart Tilman Hospital, University of Liège (B34), Department of Gastroenterology, Liège, Belgium, 9Erasme Hospital, Université Libre de Bruxelles, Department of Human Genetics, Brussels, Belgium

Background

We aimed to investigate the 163 known loci and top SNP associated with inflammatory Bowel Disease (IBD) in a non- Caucasian Moroccan IBD cohort.

Methods

We genotyped 549 non- Caucasian Moroccan individuals with 285 IBD patients (211 Crohn's disease (CD), 63 Ulcerative colitis (UC) and 11 Indeterminate colitis (IC)) and 264 controls on custom designed Immunochips from ILLUMINA®. We considered the 163 loci and top SNPs associated with IBD in Caucasian individuals and negative controls matched for the minor allele frequencies (MAF). After quality controls, association analysis was done using PLINK and population stratification was corrected using the first five principal components as covariates. Simulation tests with random groups of SNP (outside the 163 loci and matched for MAF) and regions (outside the 163 IBD loci and matched for the number of independent tests) were used to test the significance of the results.

Results

We identified 10 regions significantly associated with IBD and UC (Table1).

 

“Table 1: ten top SNP/loci significantly associated with IBD and UC”

ECCOJC jju027 P705 F0001

 

Surprisingly, none of NOD2 variants were found to be associated with IBD moroccan population. Increasing the sample size of the used cohort will definitively help to detect more IBD loci/SNPs associated with Moroccan IBD.

Conclusion

This is the first genetic study conducted in a large population of Moroccan IBD patients. Ten loci/top SNP were significantly associated with IBD and UC. Interestingly, none of the NOD2 variants were found to be associated to CD/UC and Moroccan IBD.