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* = Presenting author

P723 Differential DNA flexibility for symbiotic species involved in gastrointestinal symbiotic interactions

V.-L. Héctor Gilberto*1, F.C. Gabriela2

1Universidad Nacional Autónoma de México, Faculty of Sciences, Mexico City, Mexico, 2Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán. , Departament of Gastroenterology, Mexico City, Mexico

Background

Changes in the abundance of firmicutes and bacteroidetes have been linked to dysbiosis in human disorders, to recognize how the symbiotic organism change and model this interaction is crucial. According to the recent Black Queen hypothesis, the genomic structure with a symbiotic lifestyle can be implied in events of reduction and change in genomic architecture and composition, bringing into events of reduction and public goods, this could even impact since bacterial communities and symbiosis events. The human microbiome is one of the most important event of symbiosis, with implications in health, economic impact and evolution and it is known that the Firmicutes and Bacteroidetes order is one of the most important bacterial involved in regulations and interaction with the microbiota as well as the gastrointestinal host epithelia. It is proposed that in some cases, the Firmicutes orders could even have a shared and a common signal in interactions and regulations within the human gut, however due to the black queen hypothesis it might be possible that the reduction and the genomic architecture could even be modified at symbiotic sequenced strains and types.The aim of the study was to recognized particular motifs of tandem repeats and high flexibility sequences in the chromosome of some species involved in symbiosis lifestyle for the human gut.

Methods

Using the UGENE software and R modules we compared the incidence of these sequences in complete genome sequences from the NCBI databases for Firmicutes and Bacteroidetes species involved in symbiotic lifestyle.

Results

After we compare 21 species from Bacteroidetes (10) and Firmicutes species (11), we recognize several regions of tandem repeats presented in differential proportion. We can recognize that the Firmicutes show a differential high signal of regions of DNA with higher flexibility bringing the possibility of a differential feature plasticity and recombination and possibly lateral gene transfer, bringing different general a particular configuration.

Conclusion

Firmicutes species resemble a high input into the microbiota of the human gut. however their signal and features involved in regulation can not be generalized. With this approach we can suggest that some genera of Firmicutes and their interaction and particular plasticity of Bacteroides can even being implied in regulation systems and differential response in the gut microenvironment.