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* = Presenting author

P056 Evaluation of oxidative stress in experimental model of Chron’s disease under hyperbaric oxygen treatment

F. S. Nakutis1, I. Nishitokukado1, F. M. d. Santos1, C. Ortiz-Agostinho1, V. S. Nunes2, A. Zonetti1, A. Sipahi*1

1Faculdade de Medicina da USP, Gastroenterologia Lim07, São Paulo, Brazil, 2Universidade de Medicina da USP, Laboratório Lípides, São Paulo, Brazil

Background

Knowledge about the physiopathogenesis of inflammatory bowel diseases (IBD) has evolved over the last few decades. However, although therapies have improved, 2/3 of the cases still need alternative medicines and support therapy. The constant search for alternative treatments and modalities that are more effective has brought to light some promising strategies, such as the use of hyperbaric oxygen (HBO). The use of such therapy surged rapidly in the 1990s, showing good results and few side effects, but was later ‘forgotten’ because of the efficacy shown by the use of biological therapies. This project aimed to evaluate the effects of hyperbaric oxygen (HBO) treatment in mice with chemically induced colitis, using 2,4,6 trinitrobenzene sulfonic acid 2.5% (TNBS). We evaluation the following aspects: histology; inflammatory profile through cytokines IL-4, IL-10, IL-12, IL-13, IL-17, TNF- α and interferon ɣ; as well as the activity of the antioxidant enzymes superoxide dismutase (SOD), gluthatione peroxidase (GPx) and gluthatione reductase (GR), in the bowel mice.

Methods

Male mice were divided into 6 groups as follows: TNBS, TNBS+HBO, ALCOHOL, ALCOHOL+HBO, SALINE and SALINE+HBO. During the treatment, the models were evaluated daily. Treatment with HBO was performed for 4 days.

Results

This study has shown that the HBO promoted a significant improvement on clinical status of these animals. The histological evaluation of the TNBS+HBO group presented a significant improvement when compared with TNBS group. Treatment with HBO increased the activity of the antioxidant enzymes SOD and GPx in all groups and was only significant in the groups TNBS vs TNBS+HBO, whereas differences in the activity of GR were not observed amongst the groups. Regarding the inflammatory profile, we observed that the treatment with HBO promoted the decrease of the pro-inflammatory cytokines INFɣ, IL-12, IL-17, and TNF-α, as well as the increase of anti-inflammatory ones IL-4 and IL-10, whereas IL-13 was not affected.

Conclusion

These data show, in an experimental model, the potential anti-inflammatory effect and the enhancement of the enzymatic antioxidant defences promoted by the HBO.