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* = Presenting author

P109 Effect of dysbiosis on the growth and colitis susceptibility in DSS colitis model

C. S. Eun*, D. S. Han, A. R. Lee, Y. R. Lee, H. G. Kim

Hanyang University Guri Hospital, Gastroenterology, Guri, South Korea

Background

The intestinal microbiota is important for maintaining normal physiology and energy production including growth, body temperature regulation, and reproduction throughout life. An altered intestinal microbial composition (dysbiosis) has been associated with obesity and metabolic syndrome, as well as inflammatory bowel diseases. We investigated the effects of intestinal microbiota change on the growth and colitis susceptibility in dextran sodium sulfate (DSS) colitis murine model.

Methods

We assigned 4-week-old C57BL/6 mice to 2 groups: a group treated with vancomycin (500 mg/L for 4 weeks) plus subsequent 1.7% DSS (5 days) and a DSS-treated group. Clinical activities including weight change and histologic findings of colonic segments were examined. Proinflammatory cytokine (IL-12p40, IL-6, and IFN-γ) levels were measured by ELISA in the supernatants of colonic tissue explants. To characterise the change of intestinal microbiota, DNA extraction from the sequential faeces and quantitative polymerase chain reaction (PCR) for Firmicutes, Bacteroidetes, and E. coli were performed.

Results

During vancomycin treatment, a significant weight gain was observed in the vancomycin-and-DSS-treated group compared with vancomycin-un-treated group. In microbiological assay, phylum Firmicutes and phylum Bacteroidetes were significantly decreased, and E. coli species was significantly increased in the vancomycin-treated group. After DSS treatment, histological inflammation and proinflammatory cytokine levels were increased in the vancomycin-and-DSS-treated group compared with DSS-treated group.

Conclusion

The change of intestinal microbiota through vancomycin administration affects the growth and colitis susceptibility in a DSS colitis murine model.