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P180 The activity of glutathione peroxidase and superoxide dismutase in the blood serum and saliva in patients with Crohn’s disease

K. Szczeklik1, R. Domagała-Rodacka2, P. Mach3, D. Cibor*2, J. Pytko-Polończyk1, T. Rodacki4, D. Owczarek2

1Jagiellonian University Medical College, Department of Integrated Dentistry, Kraków, Poland, 2Jagiellonian University Medical College, Department of Gastroenterology and Hepatology, Kraków, Poland, 3University of Duisburg-Essen, Department of Gynaecology and Obstetrics, Essen, Germany, 4Jagiellonian University Medical College, Department of Food Chemistry and Nutrition, Kraków, Poland

Background

Crohn’s disease (CD) is a chronic inflammatory condition with uncertain aetiology. Dysfunction of immunoregulatory factors and overproduction of reactive oxygen species (ROS) may contribute to the destruction of the gastrointestinal tract. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) are involved in protection of cells from the damaging effects of ROS.

The aim of this study was to assess the activity of SOD and GPx as oxidative stress markers in blood serum and saliva of patients with CD and correlate the activity of SOD and GPx with the disease activity and selected inflammatory markers (leukocytes, platelets, and C-reactive protein [CRP]).

Methods

Enrolled in this study were 50 patients aged 18–66 with CD diagnosed based on clinical, radiological, histopathological, and endoscopic criteria, and 30 healthy subjects (control group). The disease activity was determined by the CD activity index (CDAI; CDAI ≥ 150 means active CD, and CDAI < 150 inactive CD). The blood inflammatory markers (BMI) were assessed in both groups. The samples of blood and stimulated whole saliva were collected from fasted subjects. Blood morphology and CRP level in serum were examined. SOD and GPx were assayed in serum and saliva samples by ELISA method. Results were analysed by nonparametric tests (Mann–Whitney U test and Kruskal–Wallis test).

Results

CDAI score correlated inversely with SOD in the serum (p = 0,001; R = -0,457). Mean values of serum SOD differed between patients with active or inactive CD according to CDAI (485 vs 643 U/ml, respectively, p = 0,002). The level of blood platelets in CD patients correlated inversely with SOD in serum (p = 0,005; R = -0.4). The CRP level in CD group correlated inversely with SOD activity in serum (p = 0.0007; R = -0.47) and with SOD in saliva (p = 0.012; R = -0.37). Mean values of serum SOD differed between patients with or without fistulas (522 vs 606 U/ml, respectively, p = 0.028). In comparison to SOD, there were no significant differences in GPx activity in both serum and saliva in CD group. No significant differences in SOD and GPx level both in serum and saliva were found between CD remission group and the control group.

Conclusion

Changes in the SOD activity suggest that antioxidant system is involved in active CD. SOD level might be used as an inflammatory marker in CD patients.