P027 Comparing the immunological signatures of inflammatory diseases
McDonald E.*1, Wright P.1, Zangerle-Murray T.2, Jung J.1, Gaya D.3, Siebert S.1, McInnes I.1, Milling S.1
1University of Glasgow, Infection, Immunity and Inflammation, Glasgow, United Kingdom 2University of Manchester, Manchester, United Kingdom 3Glasgow Royal Infirmary, Glasgow, United Kingdom
Inflammatory bowel disease (IBD), ankylosing spondylitis (AS), psoriasis (Ps) and psoriatic arthritis (PsA) are chronic inflammatory diseases with overlapping symptoms, which are associated with similar genetic polymorphisms. We aimed to increase our understanding of the cellular pathways involved in these conditions. Using multi-parameter flow cytometry, we have characterised disease associated immunological signatures of patients with IBD, AS, Ps and PsA. These data can now be used to identify both common and unique immune pathways affected by these diseases and will be fundamental in identifying therapeutic targets in future.
25–38 blood samples were collected from patients with IBD, AS, Ps, PsA and healthy volunteers (HC). Patients receiving biologic therapy were excluded from the study.
Immunophenotyping was performed using multi-parameter flow cytometry, focussing on T cells, monocytes and dendritic cells.
We have collected samples from a total of 156 individuals (31 HC, 38 Ps, 32 AS, 30 PsA and 25 IBD). All samples have been analysed by high-density flow cytometry, generating data on 74 pre-defined cell populations for each sample. Analysis of this dataset is ongoing, though preliminary analysis has already revealed 3 parameters that differed significantly between HC, AS and Ps cohorts.
Analysis of the dataset is continuing, and is being integrated with the clinical data from the donors. We anticipate that this will reveal more of the cell populations, and important cellular activation pathways that are involved in the pathogenesis of these chronic inflammatory conditions.