P044 Therapeutic effects of mesenchymal stem cells induced regulatory dendritic cells on chronic colitis model in mice
Kim H.-S.*1, Jo H.1, Eom Y.W.2, Cho M.Y.3
1Yonsei University Wonju College of Medicine, Internal medicine, Wonju, South Korea 2Yonsei University Wonju College of Medicine, Cell Therapy and Tissue Engineering Center, Wonju, South Korea 3Yonsei University Wonju College of Medicine, Pathology, Wonju, South Korea
Mesenchymal stem cells (MSCs), emerging cell therapeutics for inflammatory bolwel disease (IBD), have demonstrated immunomodulatory effects by interactions between T cells and dendritic cells (DCs). In this study, we investigated whether MSCs could differentiate mature DCs (mDCs) into a distinct regulatory DCs and these DCs could alleviate inflammation through a T cell modulation in DSS induced chronic colitis mouse model.
DCs were derived from mouse bone marrow and different subsets of DCs were characterized by fluorescence-activated cell sorting (FACS). Quantitative polymerase chain reaction (q-PCR) for anti-inflammatory (interleukin (IL)-10 and transforming growth factor (TGF)–β) and pro-inflammatory cytokines (interferon (IFN)-γ, tumor necrosis factor (TNF)-α and IL-6) were performed in both
MSC-induced DCs expressed CD11c+ CD80lo CD86locell surface marker. These DCs secreted high levels of anti-inflammatory cytokines, whereas production of pro-inflammatory cytokines were diminished
These results showed that MSCs induced DCs alliviated chronic colitis by promotin g Treg cells differentiation and the secretion of anti-inflammatory cytokines, providing potential application of MSC-induced DCs in the treatment of IBD.