P143 From biopsies to fecal samples: challenging
Faecalibacterium prausnitzii and related phylogroups as potential biomarkers for IBD
Amoedo J.*1,2, Serra-Pagès M.2, Serrano M.2, Bahí A.3, Malagόn M.3, Gilabert P.4, Guardiola J.4, Busquets D.3, Aldeguer X.3, Garcia-Gil J.1,2
1Universitat de Girona, Microbiologia, Girona, Spain 2GoodGut SL, Girona, Spain 3Institut de Investigaciό Biomèdica de Girona, Girona, Spain 4Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Spain
Both, Faecalibacterium prausnitzii (Fpra), and Fpra phylogroups (PGH-I and PHG-II), combined with
A Spanish cohort consisting of 23 IBD (10 CD and 8 UC) and 12 H was enrolled. Sixty seven faecal samples (26 CD, 30 UC and 11 H) fecal samples were obtained during their treatment. Fpra total, PHG-I, PHG-II and Eco abundances were quantified by qPCR.
Fpra and PHG-II were less abundant in IBD patients, compared with healthy subjects (p=0.046 and p=0.009, respectively). In turn, abundance of Ecoli was higher in IBD patients (p=0.007) (with sensitivities and specificities between 60%-85%). As compared to H, CD patients displayed lower abundance of Fpra l, PHG-I and PHG-II (p=0.014, 0.045 and 0.004) and higher of Eco (p=0.001). Finally, PHG-II loads were lower for Fpra and higher for Eco in samples from UC patients (p=0.050 and p=0.047).
Concerning disease localization, significant differences were observed in CD. Fpra and PHG-I abundances were lower in the colon (p=0.046 and p=0.026), whereas PHG-II was significantly higher in the ileum (p=0.032).
UC and CD with colon affectation could also be distinguished; a lower abundance of PHG-I and Eco (p=0.020 and p=0.048) was observed in samples of colon CD, with sensitivities and specificities between 70%-100%.
Fecal loads of total Fpra and related phylogroups correlate with those describe on biopsy samples.
Ecoli and PHG-I seem to be good markers to discriminate between patients with UC and CD with colonic affectation.
 Mireia Lόpez-Siles, (2016), Changes in the Abundance of