P201 A faecal bacterial markers correlates with Calprotectin for IBD activity monitoring?
Amoedo J.*1,2, Serra-Pagès M.2, Serrano M.2, Bahí A.3, Malagόn M.3, Gilabert P.4, Guardiola J.4, Busquets D.3, Garcia-Gil J.1,2, Aldeguer X.3
1Universitat de Girona, Microbiologia, Girona, Spain 2GoodGut SL, Girona, Spain 3Institut de Investigaciό Biomèdica de Girona, Girona, Spain 4Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Spain
Calprotectin (CP) faecal concentration is widely used as a non-invasive marker of inflammation of the intestinal mucosa, to assess the disease activity. In faecal samples, the abundance of
A Spanish cohort consisting of 23 IBD (10 CD and 8 UC) and 12 H was enrolled. Sixty seven faecal samples (26 CD, 30 UC and 11 H) were obtained during treatment. Fpra, PHG-I, PHG-II and Eco abundances were determined by qPCR, and CP levels, using values above and below 250μg/g for active and inactive disease, respectively.
The abundances of Fpra in CD and UC were lower in group CP >250μg/g compared to CP <250μg/g (p=0.024 and p=0.019, respectively). The abundance of PGH-II was lower in CD samples with CP >250μg/g (p=0.008), while PGH-I was less abundant in samples of active UC (p=0.002). No significant differences were found for Eco.
There was a significant inverse correlation between abundances of Fpra and the level of CP for both IBD (p=0.038 for CD and 0.035 for UC). CP was also inversely related to both, PHG-I and PHG-II in UC (p=0.013) and in CD (p=0.050), respectively.
The abundance of Fpra could be an accurate general discriminator of active disease, for IBD. In addition, PHG-I and PHG-II can be used as specific indicators of active disease in CD and UC, respectively.