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* = Presenting author

P201 A faecal bacterial markers correlates with Calprotectin for IBD activity monitoring?

Amoedo J.*1,2, Serra-Pagès M.2, Serrano M.2, Bahí A.3, Malagόn M.3, Gilabert P.4, Guardiola J.4, Busquets D.3, Garcia-Gil J.1,2, Aldeguer X.3

1Universitat de Girona, Microbiologia, Girona, Spain 2GoodGut SL, Girona, Spain 3Institut de Investigaciό Biomèdica de Girona, Girona, Spain 4Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Spain

Background

Calprotectin (CP) faecal concentration is widely used as a non-invasive marker of inflammation of the intestinal mucosa, to assess the disease activity. In faecal samples, the abundance of Faecalibacterium prausnitzii (Fpra) and Fpra phylogroups (PGH-I and PHG-II), combined with Escherichia coli (Eco) seems to be an accurate biomarker to distinguish healthy (H) and diseased and also between disease locations according to a data also presented in this meeting by our group. The purpose of this study was to analyse the co-variation between these indicators and the disease activity in patients with CD and UC, and to determine its usefulness to discriminate between active and inactive disease.

Methods

A Spanish cohort consisting of 23 IBD (10 CD and 8 UC) and 12 H was enrolled. Sixty seven faecal samples (26 CD, 30 UC and 11 H) were obtained during treatment. Fpra, PHG-I, PHG-II and Eco abundances were determined by qPCR, and CP levels, using values above and below 250μg/g for active and inactive disease, respectively.

Results

The abundances of Fpra in CD and UC were lower in group CP >250μg/g compared to CP <250μg/g (p=0.024 and p=0.019, respectively). The abundance of PGH-II was lower in CD samples with CP >250μg/g (p=0.008), while PGH-I was less abundant in samples of active UC (p=0.002). No significant differences were found for Eco.

There was a significant inverse correlation between abundances of Fpra and the level of CP for both IBD (p=0.038 for CD and 0.035 for UC). CP was also inversely related to both, PHG-I and PHG-II in UC (p=0.013) and in CD (p=0.050), respectively.

Conclusion

The abundance of Fpra could be an accurate general discriminator of active disease, for IBD. In addition, PHG-I and PHG-II can be used as specific indicators of active disease in CD and UC, respectively.