P790 Microbiota profile in pediatric IBD: correlations with phenotype and disease activity
Gatti S., Annibali R., Del Baldo G., Franceschini E., Lionetti E., Albano V., Galeazzi T., Catassi C.
Università Politecnica delle Marche, Department of Pediatrics, Ancona, Italy
Many studies have shown that in active IBD there is a dysbiosis, which could be a cause for a disturbed epithelial barrier function. The pediatric IBD patient, especially at onset of disease, offers a unique opportunity to investigate pathogenetic and particularly microbiological aspects of the disease.
Children with IBD were enrolled in the period 2013–2014, both patients at diagnosis (treatment naïve) and during follow-up.
Stool samples were collected and immediately frozen. The microbiota composition was analyzed through amplification of the V3-V4 regions of the 16S rRNA gene and subsequently sequencing on Illumina MiSeq using the 300 bp paired-end protocol. Microbiological data were correlated to clinical (disease type, phenotype, activity) and laboratory parameters (fecal calprotectin, inflammatory markers). In a subgroup of Crohn's disease (CD) patients microbiota was analyzed before and after a course of exclusive enteral nutrition (EEN).
In the study period 16 IBD patients were enrolled (median age 12.5 years), 9 with Ulcerative Colitis (UC), 7 with CD. A total of 28 samples was collected and analyzed. The total number of sequences written was 348681 and the total number of input sequences was 94760274. After filtration the median sequence length was 318 bp (range: 214–29867 bp). Comparison between UC and CD samples revealed significance in phylum level diversity for the Firmicutes phylum (p=0.046), more represented in CD than UC. No statistical difference was found comparing active UC versus inactive UC and active CD versus inactive CD at phylum level. At species level,
The apparent increase in Firmicutes in CD subjects compared to UC subjects is an interesting result, worth of further exploration. The increased representation of