P034 A dietary fibre intervention shapes the microbiome towards an anti-inflammatory tone
S. J. Reider*1,2, S. Moosmang3, J. Tragust1, L. Trgovec-Greif4, S. Tragust5, N. Przysiecki1, S. Sturm3, H. Tilg2, T. Rattei4, H. Stuppner3, A. R. Moschen1,2
1Medical University Innsbruck, Christian Doppler Laboratory for Mucosal Immunology, Innsbruck, Austria, 2Medical University Innsbruck, Department for Internal Medicine I – Gastroenterology, Hepatology, Endocrinology and Metabolism, Innsbruck, Austria, 3University of Innsbruck, Institute of Pharmacy / Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI), Innsbruck, Austria, 4University of Vienna, Division of Computational Systems Biology, Department of Microbiology and Ecosystem Science, Vienna, Austria, 5University Halle, Institute of Biology – General Zoology, Halle (Saale), Germany
The intestinal microbiome in IBD shows characteristic changes already early in the course of disease. These include enrichment of
A clinical trial including 19 healthy volunteers (8 males, 11 females) was performed. Stool, serum and urine samples were collected weekly for 9 weeks allowing every study participant to serve as their own control. The study included 3 periods: a 3-week baseline, a 3-week intervention, and a 3-week washout phase. During the intervention phase, participants received daily dosing of 5 g PHGG for 3 days followed by 10 g PHGG for 4 days in the first week, proceeding with 2 weeks of 15 g PHGG per day. A medical and nutritional history was taken for every participant at baseline, questionnaires on abdominal symptoms were completed weekly and stool habits were recorded daily using the Bristol Stool Chart. Microbiome structure was assessed by 16S metagenomics using both V1-V3 and V3-V4 regions and Tax4Fun was used to estimate functional profiles from taxa abundance. Faecal metabolomics were studied by nuclear magnetic resonance spectroscopy (NMR). Metagenomic and metabolomic data were linked using sparse regression matrices and analysis of co-occurrence/-exclusion.
PHGG increased stool frequency and reduced stool consistency. This laxative effect was more pronounced in males than females and persisted during the washout phase. PHGG decreased α diversity during intervention, but this effect did not persist. Β-diversity was not different between study periods but taxa changing significantly under PHGG treatment were detected: PHGG was associated with reduction in certain
This study shows that a dietary intervention with PHGG induces beneficial changes of intestinal microbial composition and function along with changes in microbiota-derived metabolites. PHGG supplementation could be one way to attenuate IBD associated changes of the microbiome. We plan to investigate these effects by additional experiments in models of intestinal inflammation.