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P047 Neuroendocrine cells densities alterations in colonic mucosa of patients with inflammatory bowel disease

C. Meianu*1, G. Becheanu1, C-M. Preda1, D. Istratescu1, C-A. Ciora1, M. Manuc1, L. Tugui1, A-C. Andrei1, M. Diculescu1

1Clinic Fundeni Institute- Gastroenterology and Hepatology, Bucuresti, Romania


Several published studies on human and animal models showed increased densities of NEC in colonic mucosa of inflammatory bowel disease (IBD) colitis compared with non-IBD controls.

The aim of our study is to determine de NEC densities in colonic mucosa of patients with IBD in our Department.


Colonic biopsies from 11 patients with IBD and 11 patients screened for colorectal cancer were evaluated histopathological with haematoxylin–eosin staining and immunochemical with chromogranin A (CgA) and synaptophysin antibodies (Syn). We assessed the number of NEC by manual counting at optic microscope on 10 oriented crypts and 20 transverse sectional crypts.


In IBD group NEC had a patchy and superficial distribution, organised in groups or nodules of 3 to 6 hyperplastic cells/crypt with a mean density of 3.16 CgA positive and 2.54 Syn postive NEC/crypt in IBD group compared with 1.7 CgA positive and 1.28 Syn positive NEC/crypt in non-IBD controls; p = 0.0001, p = 0.002.

When compared with IBD duration, NEC densities decreased with IBD evolution so that in patients with IBD duration between 1 and 5 years mean NEC densities were 3,4 NEC/crypt (CgA) and 2.8 NEC/crypt (Syn) compared with 2.76 NEC/crypt and 1,72 NEC/crypt, respectively in patients with disease evolution longer than 5 years; p = 0.19, p = 0.14.

There are no significant differences between NEC distributions in active vs. inactive disease with a mean density of 2,3NEC/crypt (CgA) and 3 NEC/crypt (Syn) in active IBD colitis and 3 NEC/crypt (CgA) and 3.5 NEC/crypt (Syn) in inactive colitis; p = 0.1 and 0.2, respectively.


Our study showed an increased density of CgA and Syn positive NEC in patients with IBD. We observed a decreased in NEC densities with IBD evolution possibly related to IBD treatment