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P201 Faecal calprotectin in healthy children: are there factors affecting levels other than age?

M. Velasco Rodríguez-Belvís*1, J. F. Viada Bris1, C. Plata Fernández2, A. García Salido3, J. Asensio Antón2, L. Palomino Pérez1, R. A. Muñoz Codoceo1

1Hospital Infantil Universitario Niño Jesús, Gastroenterology and Nutrition, Madrid, Spain, 2Hospital Infantil Universitario Niño Jesús, Clinical Analysis Department, Madrid, Spain, 3Hospital Infantil Universitario Niño Jesús, Pediatric Intensive Care Unit, Madrid, Spain

Background

Our aims were to (i) establish normal levels of faecal calprotectin (FC) in healthy children and (ii) analyse the correlation with age, gender, anthropometry, perinatal data, and the type of feeding.

Methods

Multi-centre, cross-sectional, and observational study including healthy children who attended the routine follow-up visits from the Healthy Child Program. Exclusion criteria: (i) immunodeficiency; (ii) autoimmunity; (iii) gastrointestinal disease; (iv) intake of drugs; (v) gastrointestinal symptoms; or (vi) any positive finding in the associated microbiological study. We determined FC levels (Quantum Blue® test) and performed stool cultures, parasites, rotavirus, and adenovirus detection. The statistical analysis (SPSS® software) considered a p-value of <0.05 statistically significant.

Results

We included 395 subjects (3 days to 16.9 years old); 51.6% boys (mean FC 196.8, median 86.0 µg/g) and 48.4% girls (mean FC 186.0, median 71.0 µg/g), with no significant differences (Mann–Whitney U test p > 0.05). FC values showed a non-normal distribution, with higher values in young participants (see table). A negative correlation trend was found between age and FC (Spearman's rho =−0.603, p < 0.05), as shown in the image. Weight was recorded in 389 subjects (mean 17.9 kg, 95% CI 16.4–19.6). Height was recorded in 383 children (mean 94.2 cm, 95% CI 90.4–98). BMI was calculated in 382 subjects (mean 16.4 kg/m2, 95% CI 16.1–16.6). The multi-variate analysis with age as the control variable showed no correlation with weight or BMI but revealed an inverse correlation between height and FC. We found no significant differences between the type of birth and FC (Mann–Whitney U test p > 0.05) and no correlation with gestational age or birth weight (Spearman's test p > 0.05). Type of diet (breastfeeding, bottle feeding, or mixed feeding) in subjects under 6 months of age showed no correlation with FC (Kruskal–Wallis p > 0.05).

Age groupNumber of subjectsMean FC (μg/g)10thP (μg/g)50thP (μg/g)90thP (μg/g)
< 1 month43344.3156303620
1–5 months6442476325.5993
6–11 months46167.73063488
12–23 months42217.73097533
2–3 years45116.13071271
4–7 years6489.13046163
8–11 years4685.43034.5143
12–18 years4545.2303075
Total (0–18 years)395191.63077508.4

FC levels in each age group. 10thP: 10th percentile. 50thP: 50th percentile. 90thP: 90th percentile.

Abstract P201 Figure. scatterplot showing the relationship between age and FC.

Conclusion

(i) FC values in healthy children were higher than those considered as pathological in adults. (ii) A negative correlation with age and height was observed. (iii) No correlation with gender, type of birth, gestational age, birth weight, or type of feeding in the first 6 months was found. (iv) It seems necessary to reconsider the levels of FC deemed pathological in paediatric patients by age group and further analyse the role of other factors.