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P211 Relationship between the concentrations of free sulphates and 5-hydroxyindoleacetic acid (5-HIAA) in urine for IBD patients

E. Bodriagina*1, A. Nabatov2, G. Gainullina3

1Kazan State Medical University, Hospital Therapy, Kazan, Russian Federation, 2Volga Region State Academy of Physical Culture, Sport and Tourism, Science Center, Kazan, Russian Federation, 3Kazan State Medical University, Hospital therapy, Kazan, Russian Federation

Background

Sulphates are sparingly soluble salts of sulfuric acid, the increase of which may indicate the presence in patients inflammatory bowel disease (IBD). Recent findings demonstrate a possible role of sulphated compounds in the aetiology of IBD, where the latter is characterised by specific changes in 5-hydroxytryptamine metabolism. The aim of our study was to assess the level of sulphate in the urine in patients with IBD and to study relationship between the sulphate and 5-hydroxytryptamine metabolisms.

Methods

The study included 40 patients with IBD. Urine samples from patients with ulcerative colitis (UC) and Crohn’s disease (CD) taken twice in the morning and afternoon, were used for the analysis of free sulphates and 5-hydroxyindoleacetic acid (5-HIAA) with specific detection strips and ELISA, respectively.

Results

Among 40 patients, UC was detected in 26 (65%) (10 male and 16 female), CD – in 14 (35%) (7 males and 7 females). The average age was 37.2 years. The clinical characteristics of the patients were analysed. According to the severity of disease: mild, 8 patients (20%); moderate, 17 (42.5%); severe, 15 (37.5%). Among UC patients, total colitis was observed in 15 (58%) patients, left-sided colitis in 7 (27%), proctitis in 4 (15%). Among patients with CD, colitis was observed in 6 (43%), ileocolitis in 5 (36%), terminal ileitis in 3 (21%) patients. Extraintestinal manifestations of IBD were detected in 21 (52.5%) cases, of which arthropathy in 13 (61.9%), aphthous stomatitis in 5 (23.9%), spondylitis/sacroiliitis 3 (14.2%). Complications (intestine perforation, bleeding, strictures, toxic dilatation) were identified in 7 (17.5%) patients. The average level of sulphates in patients with IBD was 746.3 ± 45.0 mg/l, while in patients with UC 690.4 ± 57.0 mg/l and in patients with CD 850.0 ± 66.9 mg/l. There were no differences between the level of sulphates in the urine of patients with UC and BK (p = 0.09). However, compared with the level of sulphate in the urine of a healthy population (<400), an increase in the sulphate content was found in both patients with UC and patients with CD. We found clear correlations (R > 0.72, p < 0.01) between the concentrations of free sulphates from the ‘morning’ urine and 5-HIAA from the ‘afternoon’ urine. CD patients demonstrated stronger (R > 0.77 vs. R > 0.72) correlation compare to UC patients in both sets of experiments.

Conclusion

Patients with IBD have a higher urine sulphate level than healthy populations, which can be useful as an indirect sign of inflammation. Our data suggest a close relationship between sulphate and 5-hydroxytryptamine metabolism.