P849 Urease-positive proteobacteria in Crohn’s disease identified by novel ex vivo mucosal microbe culture combined with metagenomic sequencing (MC-MGS): the ENIGMA study
E. M. Berendsen*1,2, E. C. Hoedt1,2,3, J-J. Teh1,2, J. Zhang4,5, F. Zhang4,5, Q. Liu4,5, A. L. Hamilton6, J. Ching4,5, J. J. Sung4,5, J. Yu4,5, S. C. Ng4,5,7, M. A. Kamm6, M. Morrison1,2
1The University of Queensland Diamantina Institute, Faculty of Medicine, Brisbane, Australia, 2Translational Research Institute, Brisbane, Australia, 3University College Cork, APC Microbiome Ireland, Cork, Ireland, 4The Chinese University of Hong Kong, Department of Medicine and Therapeutics, Hong Kong, Hong Kong, 5The Chinese University of Hong Kong, LKS Institute of Health Sciences, Institute of Digestive Disease and State Key Laboratory of Digestive Diseases, Hong Kong, Hong Kong, 6The University of Melbourne and St Vincent's Hospital, Melbourne, Department of Medicine and Department of Gastroenterology, Melbourne, Australia, 7The Chinese University of Hong Kong, Centre for Gut Microbiota Research, Hong Kong, Hong Kong
Longitudinal 16S analysis of Australian Crohn’s disease patients suggest that the presence of
Anastomotic biopsies from 5 Crohn’s disease patients 6 months post-surgery, were stored in RNA later for DNA extraction. Matched biopsies stored in an anaerobically prepared glycerol buffer underwent microbe culture with a habitat-simulating medium (37ºC, 24 h). Total and microbe enriched biopsy DNA, as well as MC-DNA, was sequenced to a depth of 3 gbp as 150 bp paired-end reads with the Illumina NextSeq500 platform. In addition to metagenome-assembled genomes produced using MetaBAT,3 urease-producing (and other) bacteria were isolated using selective media under aerobic and anaerobic conditions.
MC-MGS produced 16 metagenome assembled genomes representing a broad diversity of bacteria representing both facultative and fastidious anaerobes, including members of the
Urease-positive bacteria, principally members of the
1. Wright EK, et al., Microbial factors associated with postoperative Crohn’s disease recurrence.
2. Ni J, et al. A role for bacterial urease in gut dysbiosis and Crohn's disease.
3. Kang DD, Froula J, Egan R,
- Posted in: Poster presentations: Microbiology (2019)