- To identify an epigenetic signature in human adipose stem cells (hASCs) isolated from CD patients with different clinical activity, and to compare this signature with that of hASCs from healthy donors (study of epigenetics).
- Comparative DNA methylome analysis in hASCs isolated from the included population [Infinium Human-Methylation EPIC (850K) BeadChip; Illumina, Inc.].
- Transcriptomic study in hASCs isolated from the included population (Illumina HiSeq 2500 system).
- Bioinformatic analysis and data integration.
We have already finished the hASC characterisation and culture. We are planning to send the samples from September to the Genomics Service of Barcelona for the purposes of DNA methylation analysis and transcriptome arrays. We expect to have the raw data by November, and will then start with the bioinformatics analysis.