P039 Regional distribution of TCR Vδ1 and TCR Vδ2 cells in healthy and inflamed mucosa of inflammatory bowel disease

E. Tristan, A. Carrasco, A. Martín-Cardona, Y. Zabana, M. Aceituno, D. Horta, X. Andújar, I. Salvador, C. Loras, F. Fernández-Bañares, M. Esteve

Gastroenterology Department, Hospital Universitari Mútua Terrassa, Universitat de Barcelona, Terrassa. Centro de Investigación Biomédica en red de enfermedades hepáticas y digestivas CIBERehd, Gastroenterology Department, Terrassa, Spain


Vδ1+ and Vδ2+ are TCRγδ lymphocytes of the gut intraepithelial compartment that recognise proteins without help of antigen-presenting cells. Its relevance in inflammatory bowel disease (IBD) is unknown.



To measure Vδ1+ and Vδ2+ in T-cell subtypes [CD4+, CD8+, double positive (DP,CD4+CD8+), double negative (DN,CD4CD8) and CD103+] of healthy and IBD inflammed gut mucosa.


Twenty-six active IBD patients without immunosuppressants (n = 18 Crohn’s disease (CD), eight ulcerative colitis (UC)) and 10 healthy controls (paired biopsies of ileum, right and left colon) were included. Lymphocytes were analysed with LSRFortessa cytometer. Results expressed as % median (25–75%IQI).


Healthy mucosa: reduction in ileum of total CD3+Vδ1+ due to CD3+CD8+Vδ1+ and CD3+DN_Vδ1+

Healthy gutIleumRight colonLeft colonp-value
CD3+Vδ1+2,6 (1.2–5.7)11.0 (7.9–16.1)7.1 (5.1–9.6)0.001
CD3+CD8+Vδ1+2.7 (1.3–5.6)13.4 (7.4–18.1)10.6 (6.3–15.6)0.005
CD3+DN_Vδ1+29.8 (17.8–39.4)55.5 (37.7–72.4)51.2 (45.5–67.7)0.003

Ileal CD vs. ileal control: CD3+DN_Vδ2+ reduction and increase of Vδ1 and Vδ2 CD4+.

CD3+DN_Vδ2+5.5 (1.9–9.7)15.9 (9.9–36)0.021
CD3+Vδ2+DN44.9 (27.1–56.9)69.60 (52.9–73.5)0.027
CD3+Vδ1+CD4+6.1 (2.7–16.8)1.8 (0.5–4.0)0.043
CD3+Vδ2+CD4+20.8 (6.6–45.3)2.5 (0–5.6)0.003

Colonic CD, UC vs. control: CD3+Vδ1+ decrease in CD and UC, and decrease of their subsets CD3+CD8+Vδ1+ and CD3+DN_Vδ1+. CD103+ and CD103 showed specular behaviour with CD3+DN_Vδ1+CD103+ and CD3+CD8+Vδ1+CD103+ decrease and CD3+DN_Vδ1+CD103 and CD3+CD8+Vδ1+CD103 increase both in UC and CD. A similar effect was observed for CD103+ and CD103 of CD3+DN_Vδ2+. CD3+Vδ1+CD4+ was increased in UC and ileal CD.

CD3+Vδ1+2.1 (1.6–3.0)2.8 (1.9–20.6)7.1 (5.0–9.6)0.002
CD3+CD8+Vδ1+2.3 (1.1–5.8)3.7 (2.8–23.7)10.6 (6.4–15.6)0.013
CD3+DN_Vδ118.9 (16.2–27.5)28.4 (20.5–61.2)51.2 (45.5–67.8)0.001
CD3+CD8+_Vδ1+CD103+49.9 (17.2–60.9)45.0 (13.3–84.5)93.8 (87.7–98.4)0.002
CD3+CD8+Vδ1+CD10350.0 (32.1–82.8)55.0 (15.5–86.7)6.1 (1.6–12.3)0.003
CD3+DN_Vδ1+CD103+7.4 (2.9–14.9)48.5 (16.4–82.3)91.3 (76.3–96.1)0.001
CD3+DN_Vδ1+CD10392.5 (85.1–97.1)51.5 (17.7–83.6)8.7 (3.8–23.6)0.001
CD3+DN_Vδ2+CD103+6.5 (0.9–8.8)4.2 (0–30.0)59.8 (18.5–67.2)0.011
CD3+DN_Vδ2+CD10393.5 (91.2–99.03)72.2 (33.8–97.9)37.5 (27.5–52.2)0.004
CD3+Vδ1+CD4+4.6 (2.2–15.3)1.4 (0.7–5.8)0.6 (0.3–1.5)0.006

Reduction of Vδ1+ and Vδ2+, mainly of CD103+, may play a role in IBD pathophysiology by perpetuating inflammation. Increase of CD3+Vδ1+CD4+ in both Ileal CD and UC may compensate this decrease with a selective increase in ‘helper’ function.