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P042 Diagnostic and prognostic value of soluble adhesion molecules in patients with ulcerative colitis

Tarasova, G.(1);Dobaeva, N.(1);Iakovlev, A.(1);Volkov, A.(1);

(1)Rostov State Medical University, Gastroenterology, Rostov-on-Don, Russian Federation

Background

Experimental studies have shown that cell adhesion molecules contribute to the constant induction of pro-inflammatory cytokines (interleukin (6, 8, 10), necrosis factor of tumor-α), providing the chronicity of immune-mediated inflammation at ulcerative colitis (UC). Determination of the importance of molecules of integrin adhesion (sVCAM-1) and mucosal adressin (sMAdCAM-1) for evaluating the effectiveness of treatment of patients with UC.


Methods

119 patients with UC were examined: 17 (14.3%) with proctitis, 44 (37%) with left-sided localization, 58 (48.7%) with the total form. The comparison group consisted of 20 healthy volunteers. Determination of serum sVCAM and sMAdCAM was carried out by quantitative enzyme-linked immunosorbent assay (ELISA) based on a system for multiplex analysis using flow fluorimetry — Luminex MAGPIX (USA), ICAM-1 kit (Cusabio, USA). The indicators were assessed before the start of the basic therapy course, in accordance with national recommendations, and 12 weeks after its completion.

Results

In patients with left-sided and total UC, a 3.2 and 4.7-fold increase in sVCAM expression and a 2.8, 3.6-fold increase in sMAdCAM expression was recorded, averaging: 386.6 + 21.2 ng/ml, 623.4 + 11.1 ng/ml; 116.5 + 13.6 ng/ml, 193.8 + 15.2 ng/ml, respectively (p <0.001). In the group with rectal lesions, there was a moderate increase in the expression of adhesion molecules: 157.1 + 11.3 ng/ml, 84.5 + 18.8 ng/ml, respectively (p<0.07). Against the background of a 12-week treatment course, clinical and endoscopic remission was recorded in 84 (70.6%) patients. In the groups of patients with left-sided and total UC in the drug remission phase, there was a decrease in the expression of sVCAM (194.6 + 9.2 ng/ml, 236.7 + 14.1 ng/ml) and sMAdCAM (72.4 + 8.1 ng/ml, 98.2 + 9.7 ng/ml), respectively (p <0.02). In cases with UC activity, increased levels of adhesion molecules persisted.

Conclusion

The soluble adhesion molecules of sVCAM and sMAdCAM are modern markers of inflammation that can be used for assessing the effectiveness of course therapy of UC.

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