P079 The expression of some circulating micro RNAs and serum Vitamin D levels in IBD patients
Atanassova, A.(1);GeorgievaPhD, A.C.(1);
(1)Medical university- Varna- Bulgaria, University Hospital St. Marina- Clinic of Gastroenterology, Varna, Bulgaria;
Serum miRNAs are secreted during the inflammation in the gastrointestinal tract and therefore could affect the regulatory functions of vitamin D and its signal pathways negative feedback 1,2,3. Additional tests are therefore needed to discover the correlation between the levels of vitamin D and the circulating miRNAs in patients with IBD.
The aim of this study is to assess the correlation between the serum levels of vitamin D and the expression of some miRNAs (Hs_miR-28_1, Hs_miR- 29c_1, Hs_miR-96_1, Hs_miR-191_1, Hs_miR-451_1, Hs_miR-142-5p_1, Hs_miR-199a_1, Hs_miR-363_1, Ce_miR39_1, Hs_miR-144_4, Hs_miR-142-3p_2, Hs_miR-155_2 , Hs_miR-16_2, Hs_RNU6-2_11, Hs_miR-1228-2p_1) in a total of 50 patients with IBD: 27 with CD and 23 with UC. Serum levels of 25(OH)D were measured and correlated with serum CRP, FCP, IBD activity and the expression of different miRNAs. Correlation of levels of miRNAs with some clinical factors as 25(OH)D deficiency, treatment with corticosteroids or anti-TNF antibodies was also done.
There is a significant difference (р=0.007) between the serum Vitamin D levels in patients with active disease (39.83 nmol/L ± 15.32 nmol/L) and those in remission (54.19 nmol/L ± 18.54 nmol/L). IBD activity is a risk factor for a decrease in the serum Vitamin D levels (OR=5.77 (1.552-21.503; p<0.01).
We found a weak inverse proportional correlation between levels of CRP and Vitamin D (r=-0.114;p<0.05). The correlative analysis found an inverse proportional weak to moderate correlation between the levels of FCP and Vitamin D (r=-0.259; p<0.01). The expression of miRNA-28_1 and miRNA-1228-3p_1 is increased in patients who normalized their Vitamin D levels. All others miRNAs have a higher expression in Vitamin D (25(OH)D) deficiency.
In Vitamin D (25(OH)D) deficit, the expression levels of all tested miRNAs were elevated if the patients received treatment with corticosteroids or monoclonal anti-TNF antibodies.
There is a correlation between the serum expression of different miRNAs and the Vitamin D levels in patients with IBD. Prospective cohort trials may identify the precise role of vitamin D as immune regulator as well as its relation to the expression of some miRNAs, participating in the processes of the pathogenesis, the optimization of the treatment and monitoring of the effect of different therapeutic regimens as a part of the personalized management.