P159 Faecal S100A12 as a non-invasive marker of inflammatory activity in Inflammatory Bowel Disease

Cenni, S.(1);Casertano, M.(2);Caprio, A.M.(2);Pacella, D.(3);Miele, E.(1);Martinelli, M.(1);Staiano, A.(1);Strisciuglio, C.(2);

(1)University of Naples "Federico II", Department of Translational Medical Science- Section of Pediatrics, Naples, Italy;(2)University of Campania "Luigi Vanvitelli", Department of Woman- Child and General and Specialist Surgery-, Naples, Italy;(3)University of Naples “Federico II”, Department of Public Health, Naples, Italy;


Background:  Calgranulin-C (f-S100A12) and zonulin are considered faecal marker of intestinal inflammation. The aim of our study was to evaluate f-S100A12 and fecal zonulin as alternative markers in the follow-up of IBD children to monitore the disease activity and to predict the clinical and endoscopic relapse, compared with FC and serum inflammatory markers.


Methods: Between September 2019 and March 2020, we prospectively enrolled all IBD pediatric patients, both Crohn’s Disease (CD) and Ulcerative Colitis (UC). S100A12, zonulin and faecal calprotectin (FC) were determined by enzyme-linked immunosorbent assay (ELISA).


Results: One hundred seventeen consecutive children, 39.3% with CD and 71 60.7% with UC were enrolled. No differences were found for FC, f-S100A12 and zonulin values in UC and CD. In both CD and UC there was a direct correlation between FC and f- S100A12 levels (r=0.54; p<0.001 and r = 0.63; p<0.001, respectively). F-S100 correlated directly with fibrinogen (r=0.32; p=0.038), platelets count (r=0.35; p=0.021), WBC (r=0.39; p=0.009) and SES CD (r=0.459, p=0.004) and inversely with hemoglobin (r= -0.40; p=0.0077), hematocrit (r=-0.43; p=0.015). In patient with UC we found a direct correlation between f-S100 and CRP (r=0.252; p=0.040) and platelet count (r=0.254; p=0.038). There was no correlation between f-S100A12 and PUCAI or PCDAI score. No association was found between Paris and fecal biomarkers evaluated both in CD and UC.  No significant correlation were found between zonulin and the different parameters studied.


Conclusion:  Our data suggest that f-S100A12 and FC but not F-zonulin are useful non-invasive biomarkers in the management of pediatric IBD in follow up and to monitor endoscopic relapse in CD patients.