P839 Analysis of mucosa-associated microbiota and serum cytokines levels in Crohn’s disease
S. Fukushima1, A. Shiotani1, H. Matsumoto1, R. Inoue2, Y. Naito3
1Kawasaki Medical School Department of Internal Medicine- Division of Gastroenterology, 1Kawasaki Medical School, Department of Internal Medicine- Division of Gastroenterology, Okayama, Japan, 2Kyoto Prefectural University, Laboratory of Animal Science as a lecturer, Kyoto, Japan, 3Kyoto Prefectural University of Medicine, Division of Gastroenterology and Hepatology, Kyoto, Japan
Background
Most studies investigated the intestinal microbiota using stool instead of mucosa-associated microbiota (MAM). We previously analysed MAM using brushing samples during colonoscopy and reported that
To investigate the diversity and characteristics of MAM in Crohn’s disease (CD) and correlations between MAM and serum levels of inflammatory cytokines.
Methods
CD patients and non-IBD controls who were planning to undergo routine colonoscopy were recruited. 2L polyethylene glycol (PEG) was used as bowel preparation for colonoscopy. Mucosal brushing samples were taken from the ileum and sigmoid colon. 16S rRNA sequencing using the MiSeq system (Illumina, San Diego, CA, USA) was performed. The V3–V4 regions of the gene encoding 16S rDNA (460 bp) were tailed PCR amplified. Processing of sequence data was analysed using QIIME version 1.8.0. Functional changes in the microbiota were evaluated using PICRUSt. Serum cytokine levels were measured using Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel Immunoassay.
Results
The participants were 15 CD patients (average age 43 years, 10 men), and 13 non-IBD controls (average age 58 years, 7 men). The CD group showed a decrease in alpha diversity compared with the non-IBD group, and a significant difference was observed between the two groups in the unweighted PCoA analysis.
Conclusion
MAM in CD group were different from those in non-IBD group, suggesting that certain bacteria may control mucosal immunity.