17December2019

ECCO Grant Study Synopsis: Carolina Serena

Carolina Serena, ECCO Grant Awardee

An integrative analysis of DNA methylation and RNA-Seq data in human adipose stem cells of Crohn’s Disease patients with different clinical activity

 

Carolina Serena
© 
Carolina Serena

Aim of Research

  1. To identify an epigenetic signature in human adipose stem cells (hASCs) isolated from CD patients with different clinical activity, and to compare this signature with that of hASCs from healthy donors (study of epigenetics).
  2. To explore the effects of DNA methylation on the regulation of gene expression in hASCs isolated from the same cohort (study of transcriptomics).
  3. Integration of data to identify the interplay between differentially methylated DNA sites and the transcriptome profile of hASCs from healthy and CD subjects that could be involved in the dysregulation of hASCs associated with CD.

Methodology

  1. To identify an epigenetic signature in human adipose stem cells (hASCs) isolated from CD patients with different clinical activity, and to compare this signature with that of hASCs from healthy donors (study of epigenetics).
  2. Comparative DNA methylome analysis in hASCs isolated from the included population [Infinium Human-Methylation EPIC (850K) BeadChip; Illumina, Inc.].
  3. Transcriptomic study in hASCs isolated from the included population (Illumina HiSeq 2500 system).
  4. Bioinformatic analysis and data integration.

Proposed timing

We have already finished the hASC characterisation and culture. We are planning to send the samples from September to the Genomics Service of Barcelona for the purposes of DNA methylation analysis and transcriptome arrays. We expect to have the raw data by November, and will then start with the bioinformatics analysis.

Posted in ECCO News, Fellowships & Grants Synopsis Reports, Committee News, SciCom, Volume 14, Issue 4

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